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          "interaction_type": "CHROMATOGRAPHIC PURITY (HPLC/UV)",
          "related_substance": {
            "uuid": "ec85539e-1646-4daa-9206-8de5cc10e24b",
            "refuuid": "7391c7ed-f634-4a95-8d80-e47ae65afc4c",
            "name": "ANABASINE",
            "unii": "LMS11II2LO",
            "linking_id": "LMS11II2LO",
            "ref_pname": "ANABASINE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "3f2c5e75-2e56-45cb-9a3c-b1f4ae2164fe",
          "type": "ACTIVE MOIETY",
          "related_substance": {
            "uuid": "bf6a2056-a5e4-40c1-85ef-94dbca3ad715",
            "refuuid": "78c74488-c8c5-41e9-9600-c41f80917780",
            "name": "NICOTINE",
            "unii": "6M3C89ZY6R",
            "linking_id": "6M3C89ZY6R",
            "ref_pname": "NICOTINE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "abccc886-f5e8-4a7f-b61c-9f43a83143f4",
          "comments": "circulating nicotine is metabolized to cotinine by cytochrome P450 and aldehyde oxidase",
          "type": "METABOLITE->PARENT",
          "interaction_type": "MAJOR",
          "references": [
            "b0714ab0-c0db-43ce-b36e-1a39b1e61b41"
          ],
          "related_substance": {
            "uuid": "f67ac1c9-dc8d-4b07-99fb-87f124c82141",
            "refuuid": "903dcef7-ced0-4513-8c63-4677f33f48cf",
            "name": "COTININE",
            "unii": "K5161X06LL",
            "linking_id": "K5161X06LL",
            "ref_pname": "COTININE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "ea4cb2f8-e217-45c2-b9fe-c6e4c8ea1476",
          "type": "SALT/SOLVATE->PARENT",
          "related_substance": {
            "uuid": "8497a6b2-8a21-48fd-9706-a43c5b4a3995",
            "refuuid": "00f5b658-5bf0-455e-87b3-d81b8e465b73",
            "name": "NICOTINE BITARTRATE",
            "unii": "R7M676M8YV",
            "linking_id": "R7M676M8YV",
            "ref_pname": "NICOTINE BITARTRATE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "4ad82676-fa02-45e4-b7b5-3fc8cabc4d24",
          "type": "SALT/SOLVATE->PARENT",
          "related_substance": {
            "uuid": "41f23b40-e6de-433e-860e-97e427a86308",
            "refuuid": "89673bef-815b-458d-9433-0b5f75dc3801",
            "name": "NICOTINE BITARTRATE ANHYDROUS",
            "unii": "7892ZN5G41",
            "linking_id": "7892ZN5G41",
            "ref_pname": "NICOTINE BITARTRATE ANHYDROUS",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "d983f606-5c94-46e5-990f-1ad0f14e0c91",
          "type": "SALT/SOLVATE->PARENT",
          "related_substance": {
            "uuid": "9d69a2c1-be9e-4172-afd2-3a7725338868",
            "refuuid": "da88589b-8ed2-4bb6-a5ea-7d0fea3e156e",
            "name": "NICOTINE DIHYDROCHLORIDE",
            "unii": "I16I55623S",
            "linking_id": "I16I55623S",
            "ref_pname": "NICOTINE DIHYDROCHLORIDE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "4eb1cac0-bfa7-49a6-85ab-810a30a521ce",
          "type": "SALT/SOLVATE->PARENT",
          "related_substance": {
            "uuid": "8a1420f7-40e1-4780-8707-c854faa9b9f7",
            "refuuid": "78bd4bb6-2ad2-45a1-9f8b-100d27ab33bc",
            "name": "NICOTINE SALICYLATE",
            "unii": "744Q1CL77H",
            "linking_id": "744Q1CL77H",
            "ref_pname": "NICOTINE SALICYLATE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "d1189f21-b0ba-4953-9801-cf8f7dba24fb",
          "type": "SALT/SOLVATE->PARENT",
          "related_substance": {
            "uuid": "1314f841-c058-42db-a2d6-c1c800fdefee",
            "refuuid": "748044cb-7bdc-465f-9f1e-b78cbc129ea3",
            "name": "NICOTINE SULFATE",
            "unii": "UX4GTH155W",
            "linking_id": "UX4GTH155W",
            "ref_pname": "NICOTINE SULFATE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "c689df9c-e2c7-487a-ab9b-4f91b15d8b4f",
          "amount": {
            "uuid": "4d5cc5dd-4b27-4a4e-ab7e-16bc4b88080f",
            "type": "RELATIONSHIP_AMOUNT",
            "units": "MOLE PERCENT OF AMOUNT ADMINISTERED",
            "high_limit": 7,
            "low_limit": 4
          },
          "comments": "in animals both N-oxide isomers form, in humans only the transisomers form.",
          "qualification": "URINE",
          "type": "METABOLITE->PARENT",
          "interaction_type": "MAJOR",
          "references": [
            "1026d7d8-bd22-467e-9319-22ae6a7f365c"
          ],
          "related_substance": {
            "uuid": "0991329a-af3a-40a2-afde-e4304b10d61c",
            "refuuid": "775cd0f9-3812-4967-9f71-47a73bfe6a31",
            "name": "NICOTINE N'-OXIDE, (1'S,2'S)-",
            "unii": "6V35J35GP5",
            "linking_id": "6V35J35GP5",
            "ref_pname": "NICOTINE N'-OXIDE, (1'S,2'S)-",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "6aaed0bb-8065-409c-a539-8a100c6cc9fa",
          "amount": {
            "uuid": "df1b82be-9f95-404e-9a64-408668ae1f70",
            "type": "RELATIONSHIP_AMOUNT",
            "average": 75,
            "units": "MOLE PERCENT OF AMOUNT ADMINISTERED",
            "high_limit": 80,
            "low_limit": 70
          },
          "comments": "75% is converted into cotintine which is further metabolized.",
          "qualification": "PLASMA; URINE",
          "type": "METABOLITE LESS ACTIVE->PARENT",
          "interaction_type": "MAJOR",
          "mediator_substance": {
            "uuid": "934cf93b-8494-4a97-86c2-e43906bc1496",
            "refuuid": "660246ec-9ab8-48a7-935b-3c32fc934075",
            "name": "CYTOCHROME P450 2A6",
            "unii": "246D9TQ2XG",
            "linking_id": "246D9TQ2XG",
            "ref_pname": "CYTOCHROME P450 2A6",
            "substance_class": "reference"
          },
          "references": [
            "1026d7d8-bd22-467e-9319-22ae6a7f365c"
          ],
          "related_substance": {
            "uuid": "3cf0c800-4cce-40f8-b893-6cb6c0bf92ec",
            "refuuid": "775cd0f9-3812-4967-9f71-47a73bfe6a31",
            "name": "NICOTINE N'-OXIDE, (1'S,2'S)-",
            "unii": "6V35J35GP5",
            "linking_id": "6V35J35GP5",
            "ref_pname": "NICOTINE N'-OXIDE, (1'S,2'S)-",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "e4abd7e0-8067-461c-9587-91e4d26c41df",
          "amount": {
            "uuid": "7fb9c708-6ec1-4a91-9141-21a39edf81ee"
          },
          "qualification": "PLASMA; URINE",
          "type": "METABOLITE->PARENT",
          "references": [
            "eb4b8ff0-1261-4131-b406-e29fdaaa0b3c"
          ],
          "related_substance": {
            "uuid": "68b4bd18-9925-46bf-923c-c091f3e00d8b",
            "refuuid": "cedac6f5-3ac2-4c32-8d09-dd6f57962516",
            "name": "3'-HYDROXYCOTININE, TRANS-",
            "unii": "H7LH47Y29A",
            "linking_id": "H7LH47Y29A",
            "ref_pname": "3'-HYDROXYCOTININE, TRANS-",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "cdcde847-92f2-4110-a4c4-2733f76adc08",
          "comments": "Nicotine (mg/cigarette) \nDomestic = 12.6 SD(1.6)\nImported = 11.1 SD(2.2)",
          "type": "PARENT->CONSTITUENT ALWAYS PRESENT",
          "references": [
            "35bff1e2-3b82-4e20-aa8f-880c1900aea5"
          ],
          "related_substance": {
            "uuid": "56a514ee-3359-454c-b658-c1685f77306b",
            "refuuid": "58f6d6ce-a65e-4351-b483-33cad323b1b8",
            "name": "TOBACCO LEAF",
            "unii": "6YR2608RSU",
            "linking_id": "6YR2608RSU",
            "ref_pname": "TOBACCO LEAF",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "b8c99c54-7cf8-4173-8069-688b8f4377ba",
          "type": "SUB_CONCEPT->SUBSTANCE",
          "related_substance": {
            "uuid": "9b9bac4b-c2cd-4fc3-b73b-630568ec4dda",
            "refuuid": "a571e0d9-f8f1-4334-8ec2-37a8d1e16301",
            "name": "NICOTINE BETADEX",
            "linking_id": "a571e0d9-f8f1-4334-8ec2-37a8d1e16301",
            "ref_pname": "NICOTINE BETADEX",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "fdf1b062-12df-4e42-bcdc-142f4cdcb2a5",
          "type": "SUB_CONCEPT->SUBSTANCE",
          "related_substance": {
            "uuid": "19ceb127-c8a7-454d-b1c5-5fc56d4bbda5",
            "refuuid": "f2f15f7c-e2c9-4d9c-a996-36829e2d31bb",
            "name": "NICOTINE POLACRILEX",
            "linking_id": "f2f15f7c-e2c9-4d9c-a996-36829e2d31bb",
            "ref_pname": "NICOTINE POLACRILEX",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "73198519-198b-463c-8630-396adba60609",
          "amount": {
            "uuid": "17a6c250-d325-4724-929f-7deffa80fa7e",
            "high": 5,
            "units": "%"
          },
          "type": "BINDER->LIGAND",
          "interaction_type": "BINDING",
          "references": [
            "e924036b-e0c5-eb27-246b-087dbf485e52"
          ],
          "related_substance": {
            "uuid": "2f0de71e-92e2-499f-a5c8-2e793d6b56f0",
            "refuuid": "e95d330b-4c2d-44bb-a61b-860afeb4aa30",
            "name": "PLASMA PROTEIN FRACTION (HUMAN)",
            "unii": "6D53G0FD0Z",
            "linking_id": "6D53G0FD0Z",
            "ref_pname": "PLASMA PROTEIN FRACTION (HUMAN)",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "fc0a559a-d90b-4503-907c-3d96ecb10a7d",
          "type": "METABOLITE INACTIVE->PARENT",
          "references": [
            "6f3a18bf-c788-47ab-ab7b-613dddab59eb"
          ],
          "related_substance": {
            "uuid": "b5cb8f41-2a0c-42f0-8213-47a74076ecc0",
            "refuuid": "50b69327-6b9a-4d6a-b655-a46778c81a8f",
            "name": "BA-2702",
            "unii": "OL86R2ULD8",
            "linking_id": "OL86R2ULD8",
            "ref_pname": "BA-2702",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "3b12e793-8a31-43bd-a38a-cfb04d69090f",
          "amount": {
            "uuid": "87c07bc8-cd5a-40ac-901e-e9aeafa6a0a9"
          },
          "comments": "NICOTINE IS AN ANTIHERBIVORY ALKALOID AND POTENT PARASYMPATHOMIMETIC STIMULANT AND FOUND IN PLANTS, MOSTLY THOSE IN THE NIGHTSHADE FAMILY. NICOTINE ACTS AS A RECEPTOR AGONIST AT MOST NICOTINIC ACETYLCHOLINE RECEPTORS (NACHRS), EXCEPT AT TWO NICOTINIC RECEPTOR SUBUNITS (NACHR.ALPHA.9 AND NACHR.ALPHA.10) WHERE IT ACTS AS A RECEPTOR ANTAGONIST.",
          "type": "TARGET->AGONIST",
          "related_substance": {
            "uuid": "7616e009-0100-47e4-b67f-386ce1c372f4",
            "refuuid": "15364981-17e3-4ae8-a665-8c49fe752435",
            "name": "NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7",
            "unii": "P4J1I8C6CX",
            "linking_id": "P4J1I8C6CX",
            "ref_pname": "NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "c1dc3591-22dd-4e7d-b214-25ef765a1592",
          "type": "SALT/SOLVATE->PARENT",
          "references": [
            "2280c493-bc99-95c1-e194-997956a0c163"
          ],
          "related_substance": {
            "uuid": "73a2ec68-ae64-4c2b-8df9-c4dd344d97b6",
            "refuuid": "1e327bb3-0936-4d1d-b3f6-b01ed2d1bd00",
            "name": "NICOTINE DICITRATE",
            "unii": "F0PHZ04D78",
            "linking_id": "F0PHZ04D78",
            "ref_pname": "NICOTINE DICITRATE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "5e5637d4-2e1e-442f-a79e-8c9f9231ad38",
          "type": "SALT/SOLVATE->PARENT",
          "references": [
            "2280c493-bc99-95c1-e194-997956a0c163"
          ],
          "related_substance": {
            "uuid": "b738f48b-216d-42e2-8db9-9d2254ab83c3",
            "refuuid": "5d1f3291-7111-44d6-98f7-10c3651c37c9",
            "name": "NICOTINE HEMICITRATE",
            "unii": "LSV6GBW93D",
            "linking_id": "LSV6GBW93D",
            "ref_pname": "NICOTINE HEMICITRATE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "f9d0354b-fe84-42e9-8e07-35c486125020",
          "type": "LABELED->NON-LABELED",
          "related_substance": {
            "uuid": "11d397a8-0d1c-4a6d-b689-982a650b63ef",
            "refuuid": "1d36aed4-d883-4a8a-a98b-756f4f2aef19",
            "name": "NICOTINE C-11",
            "unii": "T397A2CQJ9",
            "linking_id": "T397A2CQJ9",
            "ref_pname": "NICOTINE C-11"
          }
        },
        {
          "uuid": "8219e3e8-400f-40da-b04a-b827726569b4",
          "type": "METABOLIC ENZYME->SUBSTRATE",
          "references": [
            "8499fb2c-7a32-92e7-fcab-c4e978ddedca"
          ],
          "related_substance": {
            "uuid": "e7073c9b-cdf5-43aa-89c1-66f4946802fe",
            "refuuid": "b4089639-3c43-4cc2-9242-dfe9fabef027",
            "name": "URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE 2B10",
            "unii": "JZ7JBT2MTT",
            "linking_id": "JZ7JBT2MTT",
            "ref_pname": "URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE 2B10",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "e9bf5f10-c1cc-44aa-ac77-c84c99a97e5f",
          "comments": "The extent of Cotinine N-glucuronidation by African Americans is significantly lower than by Whites.",
          "type": "METABOLITE->PARENT",
          "mediator_substance": {
            "uuid": "13458695-8a94-4c6a-a22d-f157b20a89f1",
            "refuuid": "b4089639-3c43-4cc2-9242-dfe9fabef027",
            "name": "URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE 2B10",
            "unii": "JZ7JBT2MTT",
            "linking_id": "JZ7JBT2MTT",
            "ref_pname": "URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE 2B10",
            "substance_class": "reference"
          },
          "references": [
            "8499fb2c-7a32-92e7-fcab-c4e978ddedca"
          ],
          "related_substance": {
            "uuid": "d7df3ae3-5098-43af-9861-c4e3095aaafa",
            "refuuid": "d2a0724c-7b76-4509-863b-d681586d12e4",
            "name": "COTININE N-GLUCURONIDE",
            "unii": "E3PE1559SW",
            "linking_id": "E3PE1559SW",
            "ref_pname": "COTININE N-GLUCURONIDE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "e20c0ce6-d2a1-4a4e-bed6-27662ed49319",
          "comments": "The extent of nicotine N-glucuronidation by African Americans is significantly lower than by Whites.",
          "type": "METABOLITE->PARENT",
          "mediator_substance": {
            "uuid": "626368e4-31b1-4304-bfb0-ccd2fab6eabc",
            "refuuid": "b4089639-3c43-4cc2-9242-dfe9fabef027",
            "name": "URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE 2B10",
            "unii": "JZ7JBT2MTT",
            "linking_id": "JZ7JBT2MTT",
            "ref_pname": "URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE 2B10",
            "substance_class": "reference"
          },
          "references": [
            "8499fb2c-7a32-92e7-fcab-c4e978ddedca"
          ],
          "related_substance": {
            "uuid": "e68e81e1-1b38-46fe-8dfe-31ab022c8123",
            "refuuid": "5f112084-5a1c-409f-b9ad-8b6db14f3b23",
            "name": "NICOTINE N-GLUCURONIDE",
            "unii": "CB9VB3OLM0",
            "linking_id": "CB9VB3OLM0",
            "ref_pname": "NICOTINE N-GLUCURONIDE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "ffe9691b-9ea3-4ad4-bd99-69ec83bb4b51",
          "amount": {
            "uuid": "c0c3fccc-c9fc-4359-b1ae-d703278f14a8"
          },
          "type": "SALT/SOLVATE->PARENT",
          "references": [
            "70a6acdf-3903-4e8f-90e3-a966a14d1b31",
            "17d2a834-283e-4151-9796-58b347376fbd"
          ],
          "related_substance": {
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            "refuuid": "037317ed-edd6-425a-b0ad-03f0fa92e253",
            "name": "NICOTINE LACTATE",
            "unii": "8XAS3GPB3T",
            "linking_id": "8XAS3GPB3T",
            "ref_pname": "NICOTINE LACTATE",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "fab13639-7de9-4b07-8fa5-98ea216093f0",
          "amount": {
            "uuid": "36c97888-227b-498e-b49a-c9f2b7f202c5"
          },
          "comments": "BINDS TO NICOTINE PREVENTS ACTION",
          "type": "INHIBITOR->TARGET",
          "related_substance": {
            "uuid": "d585c35e-b1d2-41e5-b966-af42c2388a46",
            "refuuid": "6061cec4-262f-413c-9b8d-22a9db0b068b",
            "name": "ATI-1013",
            "unii": "6DX9Y74D3E",
            "linking_id": "6DX9Y74D3E",
            "ref_pname": "ATI-1013",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "815503b7-15ee-4df6-a4b0-e2cd4ff525c4",
          "amount": {
            "uuid": "fde8a633-015c-42c1-9255-287843bb786f",
            "average": 5.9,
            "units": "NANOMOLAR"
          },
          "qualification": "IC50",
          "type": "TARGET->INHIBITOR",
          "interaction_type": "BINDING",
          "references": [
            "e0c9b5b9-13e3-48c2-8596-9a1ff84117ae"
          ],
          "related_substance": {
            "uuid": "8207355f-e8c9-40c0-b333-0f8ab1e4e477",
            "refuuid": "8b580a51-e5d2-40d1-b2c1-a3552fd6262b",
            "name": "NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-4",
            "unii": "2KC7KP5BGA",
            "linking_id": "2KC7KP5BGA",
            "ref_pname": "NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-4",
            "substance_class": "reference"
          }
        },
        {
          "uuid": "4ae10dbf-7a8f-471a-824d-c500003e8294",
          "amount": {
            "uuid": "47601c2b-c01a-4989-a909-6d29df7d941b"
          },
          "comments": "Carcinogenic substance present in cigarette smoke",
          "type": "DERIVATIVE->PARENT",
          "references": [
            "35bff1e2-3b82-4e20-aa8f-880c1900aea5"
          ],
          "related_substance": {
            "uuid": "1c1a6748-edd2-4cbb-b1ab-269ab99f53cd",
            "refuuid": "2ae1d4dc-23f9-4fd9-a403-553a4efe6d20",
            "name": "N-NITROSONORNICOTINE",
            "unii": "X656TZ86DX",
            "linking_id": "X656TZ86DX",
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